This kit employs Double Antibody Sandwich Technique. The principle of Double Antibody Sandwich is based on characteristics of the tested antigen with more than two valances which can identify coated antibody and detection antibody at same time.

The Elisa Kit is an Vitro enzyme-linked immunosorbent assay for the quantitative measurement in serum, plasma, tissue lysates, cell culture supernatants and other biological fluids. The antibodies are pre-coated onto 96-well plate. Standard and samples are aspirated into the wells and factor present in a sample is bound to the wells by the immobilized antibody. The biotinylated detection antibodies are added to the wells and then followed by Wash buffer. After washing away unbound biotinylated antibody, Enzyme Conjugate working buffer is added to the wells. The wells are washed again, a TMB Solution is added to the wells and the color changes after adding acidic Stop solution. The intensity of the color is proportional to the amount of the factor bound in samples and measured at 450nm.

The specific process is as follows: